The announcement comes just hours after the club announced it was part of controversial plans to join a European Super League.
he 58-year-old had been in charge of Spurs since November 2019 after taking over from Mauricio Pochettino.
The Portuguese manager’s original deal with the north London club had been due to run until the end of the 2023 season.
In a statement, Spurs said: “The club can today announce that Jose Mourinho and his coaching staff Joao Sacramento, Nuno Santos, Carlos Lalin and Giovanni Cerra have been relieved of their duties.
With Tottenham now five points adrift of the Champions League places with six league games remaining, Daniel Levy has now made the huge call to part with the man he appointed just under 18 months ago.
It now means Tottenham have to make a decision over who will be in charge of the team for Wednesday evening’s Premier League home game against Southampton and also Sunday’s Carabao Cup final against Man City.